Co-Expression of Calcium-Dependent Protein Kinase with the Inward Rectified Cuard Cell K^+ Channel KAT1 Alters Current Parameters in Xenopus laevis Oocytes

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概要

• 論文の詳細を見る

• Increased guard cell cytosolic [Ca^<2+>]is known to be involved in signal transduction pathways leading to stomatal closure, and inhibit the inward rectifying guard cell K^+ channel KAT1. Guard cell calcium-dependent protein kinase(CDPK)has been shown to phosphorylate KAT1;such phosphorylation is known to modulate other K^+ channels involved in signal transduction cascades. The work reported here focused on demonstrating CDPK-dependent inhibition of KAT1 currents. A cDNA encoding soybean CDPK was generated and it's translation product was shown to be functional;demonstrating Ca^<2+> -dependent autophosphorylation and phosphorylation of a target protein. Ion currents were monitored using voltage clamp techniques upon expression of KAT1 in Xenopus laevis oocytes. Coexpression of recombinant CDPK with KAT1 in oocytes altered the kinetics and magnitude of induced K^+ currents;at a given hyperpolarizing command voltage, the magnitude of KAT1 currents was reduced and the half-time for channel activation was increased. This finding supports a model of Ca^<2+> -dependent ABA inhibition of inward K^+ currents in guard cells as being mediated by CDPK phosphorylation of KAT1.

• 日本植物生理学会の論文

著者

• Mercier Richard

  Department Of Plant Science U-67 University Of Connecticut

• Zhang Xiao

  Plant Science Department

• Berkowitz Gerald

  Department of Plant Science U-67, University of Connecticut

• Leng Qiang

  Department of Plant Science U-67, University of Connecticut

• Lawton Michael

  AgBiotech Center

• Leng Qiang

  Department Of Plant Science U-67 University Of Connecticut

• Berkowitz Gerald

  Department Of Plant Science U-67 University Of Connecticut

関連論文

• Co-Expression of Calcium-Dependent Protein Kinase with the Inward Rectified Cuard Cell K^+ Channel KAT1 Alters Current Parameters in Xenopus laevis Oocytes

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