Phytophthora parasitica Elicitor-Induced Reactions in Cells of Petroselinum crispum

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Cultured parsley(Petroselinum crispum)cells respond to treatment with elicitors derived from different species of the genus Phytophthora with transcript accumulation of defense-associated genes and the production of furanocoumarin phytoalexins. Pep-25, an oligopeptide fragment of a Phytophthora sojae 42-kDa cell wall protein, and a cell wall elicitor preparation derived from Phytophthora parasitica(Pp-elicitor)stimulate accumulation of the same gene transcripts and formation of the same pattern of furanocoumarins. Treatment of cultured cells and protoplasts with proteinase-digested Pp-elicitor identified proteinaceous constituents as active eliciting compounds in parsley. Similar to Pep-25, Pp-elicitor induced effluxes of K^+ and CI^- and influxes of protons and Ca^<2+>. Concomitantly, as monitored in aequorin-transgenic parsley cell lines both elicitors induced an immediate increase in the cytoplasmic Ca^<2+> concentration up to sustained levels of 175 nM(Pp-elicitor)or 300 nM(Pep-25), respectively. The signature of the Ca^<2+> response differed greatly between the two elicitors tested. Extracellular Ca^<2+> proved essential for activation of an oxidative burst, MAP kinase activity and phytoalexin production by either elicitor. While Pp-elicitor induced a qualitatively similar spectrum of defense responses as did Pep-25, elicitor-specific quantitative differences in response intensity and kinetics suggest activation of a conserved signaling cascade through separate ligand binding sites.
日本植物生理学会の論文

Phytophthora parasitica Elicitor-Induced Reactions in Cells of Petroselinum crispum

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