A STUDY ON 3-DEOXY-D-ARABINO-HEPTULOSONIC ACID 7-PHOSPHATE SYNTHASE IN HIGHER PLANTS

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Several plant tissues were assayed for DAHP synthase activity. The activity was found in most of the plants examined, and the highest activity was detected in developing mung bean plants. During the growth of mung bean shoots, the highest level of the activity on protein basis was seen 6 to 8 days after sowing. Some properties of DAHP synthase were investigated using the enzyme preparation mainly from mung bean shoots. The product of the reaction was identified as DHAP by paper chromatography of the reaction mixture and by the absorrption spectrum of the derivative formed by periodate-TBA treatment. Mg^<++> at 1-5 mM concentration increased the rate of DAHP formation three to four times, although the enzyme was still active in the absence of Mg^<++>. Optimal pH was found to be between pH 7.2 and 7.9. Relationship between the rate of DAHP formation and concomitant acid phosphate activity in enzyme preparations was discussed.
日本植物生理学会の論文