ROLE OF NUCLEIC ACID AND PROTEIN METABOLISM IN THE INITIATION OF GROWTH AT GERMINATION
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When dry decotyledonized embryos of Raphanus are supplied with water, a brief period of water absorption (phase A) is followed by a period of no fresh weight increase (phase B) which lasts for 8 hr at 30°. In this period, embryos become ready to advance into the period of fresh weight increase (phase C). When embryos were exposed to various concentrations of thiouracil or actinomycin D solution from 0 hr of water supply, increases in fresh weight and in RNA content measured at 13 hr were inhibited in parallel with each other. Chloramphenicol and puromycin inhibited the fresh weight increase without affecting the RNA increase. When embryos were exposed to thiouracil or puromycin for 2, 4 and 6 hr, beginning at 0 hr of water supply, the start of phase C delayed 2, 4 and 6 hr, respectively. When these drugs were given after phase B had progressed at least for 2 hr, the delay of the start of phase C was shorter than the period of the drug treatment. If given at the end of phase B, thiouracil and actinomycin D inhibited the incorporation of ^<14^gt;C-uracil but not the fresh weight increase, while chloramphenicol and puromycin inhibited the latter without inhibiting the former. During phase B, protein content per dry weight of embryo, did not increase, but the rate of ^<14>C-leucine incorporation increased remarkably to reach the level in phase C. Incorporation of labeled leucine was inhibited if embryos were subjected to thiouracil or actinomycin D action during phase B, but not if the drugs were given when phase B had been completed. Puromycin and chloramphenicol inhibited the incorporation whenever they were given. The increase in respiratory activity during phase B was inhibited relatively little by the above mentioned four drugs. In conclusion syntheses of RNA and protein seem to be essential for the progress of phases B and C, protein synthesis having a more direct effect.
- 日本植物生理学会の論文
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