RIBONUCLEIC ACID-POLYPHOSPHATE FROM ALGAE III. HYDROLYSIS STUDIES
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概要
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RNA-polyphosphate was isolated from synchronous Chlorella cells. After each of a series of hydrolytic treatments, RNA-polyphosphate was chromatographically analyzed by means of a two-column ion-exchange system. Alkaline hydrolysates contained primarily ribonucleotides, pyrophosphate, and tripolyphosphate. Acid hydrolysates contained ribonucleotides, purine bases, ribonucleosides, orthophosphate, and an unknown, inorganic, phosphorus-containing compound (X-P). Treatments with pancreatic ribonuclease, spleen phosphodiesterase, and yeast polyphosphatase left large amounts of RNA-polyphospshate fragments. Treatment with venom phosphodiesterase yielded a high molecular weight inorganic polyphosphate fraction free from RNA. Such material was hydrolyzed to pyro- and tripolyphosphate by potassium hydroxide, to orthophosphate and an unknown compound X-P by perchloric acid, and to ortho-, pyro-, and tripolyphosphate by hydroxylamine under ester hydrolysis conditions. Synthetic inorganic polyphosphate was stable to potassium hydroxide and hydroxylamine under the same conditions and yielded only orthophosphate upon perchloric acid hydrolysis. Both natural and synthetic polyphosphates were hydrolyzed to low molecular weight fragments by yeast polyphosphatase. The evidence at present indicates that in Chlorella polyphosphate is not a simple phosphate anhydride chain.
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