NUTRITIONAL STUDIES OF THE EDIBLE SEAWEED PORPHYRA TENERA I. THE INFLUENCE OF DIFFERENT B_<12> ANALOGUES, PLANT HORMONES, PURINES AND PYRIMIDINES ON THE GROWTH OF CONCHOCELIS
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The red alga Porphyra tenera has been obtained in axenic culture by the "dip and drag" technique in an agarized medium containing antibiotics. In the axenic culture, the Conchocelis of P. tenera needs vitamin B_<12> for growth. The addition of other vitamins does not increase growth further. The pattern of specificity is similar to that of Escherichia coli 113-3. Factor B, pseudovitamin B_<12> and Factor Z_2 support as much growth as vitamin B_<12>, while 2-methylmercaptoadenine and 5-methyl benzimidazole cobalamine increase the growth more than B_<12>. All the analogues containing benzimidazole can replace B_<12>. Kinetin, adenine, indoleacetic acid and especially gibberellic acid increase growth. Three purines, (xanthine, hypoxanthine and guanine) and three pyrimidines (uracil, methylcytosine and thymine) also promote growth in the presence of vitamine B_<12>.
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- NUTRITIONAL STUDIES OF THE EDIBLE SEAWEED PORPHYRA TENERA I. THE INFLUENCE OF DIFFERENT B_ ANALOGUES, PLANT HORMONES, PURINES AND PYRIMIDINES ON THE GROWTH OF CONCHOCELIS