carpel, a New Arabidopsis Epi-Mutant of the SUPERMAN Gene : Phenotypic Analysis and DNA Methylation Status
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概要
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The carpel (car) mutation affects the morphology of reproductive organs in Arabidopsis thaliana.car flowers have an increased number of carpels, on average 2.7±0.8 instead of two in the wild type. Through allelism test with fon1-3 and analysis of the methylation state of the SUPERMAN (SUP) gene in car mutants, we show that car is an epi-mutation of SUP. The methylation pattern of car is clearly distinct from that of fon1-3, another epimutation of the SUP gene. Methylation was found predominantly in Cp(A/T)p(A/G) triplets and in CpG pairs. We suggest that that the extensive SUP methylation in car has arisen from an abundant methylation of a single CpG site that was already present in abscisic acid-insensitive (abi3-4) mutants, from which car was segregating.
著者
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Montagu Marc
Laboratorium voor Genetica
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Montagu Marc
Laboratorium Voor Genetica Departement Plantengenetica Vlaams Interuniversitair Instituut Voor Biote
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Rohde Antje
Laboratorium Voor Genetica Departement Plantengenetica Vlaams Interuniversitair Instituut Voor Biote
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Boerjan Wout
Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechno
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Grunau Christoph
Abteilung Genomanalyse, Institut fur Molekulare Biotechnologie
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Beck Lode
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Bio
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Rosenthal Andre
Abteilung Genomanalyse, Institut fur Molekulare Biotechnologie
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Beck Lode
Laboratorium Voor Genetica Departement Plantengenetica Vlaams Interuniversitair Instituut Voor Biote
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Boerjan Wout
Laboratorium Voor Genetica Departement Plantengenetica Vlaams Interuniversitair Instituut Voor Biote
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Rosenthal Andre
Abteilung Genomanalyse Institut Fur Molekulare Biotechnologie
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Grunau Christoph
Abteilung Genomanalyse Institut Fur Molekulare Biotechnologie
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De Beck
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent
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