Expression of the Auxin-Inducible GH3 Promoter/GUS Fusion Gene as a Useful Molecular Marker for Auxin Physiology
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概要
- 論文の詳細を見る
Expression of the GH3 promoter/GUS reporter (GH3/GUS)gene in transgenic tobacco is silent in most vegetative tissues and organs, but can be specifically induced by auxin in all cell types throughout the plant, indicating that auxin is a limiting factor for activation of the GH3/GUS gene. In the absence of exogenous auxin, expression of the GH3/GUS gene either correlates with physiological events which are presumably mediated by auxin or occurs in cells where relatively high levels of endogenous auxin are expected. For example, the GH3/GUS gene is expressed in dormant lateral buds, tips of adventitious roots, and stem parenchyma cells where adventitious roots are initiating. The GH3/GUS gene is activated at the cut surface nearest the tip of a leaf from which a strip of tissue has been excised, and in the basal end of an excised shoot segment. Also, the GH3/GUS gene is expressed predominantly in the lower side of a gravistimulated shoot and in the dark side of a unilateral light-stimulated shoot. Furthermore, the expression pattern of the GH3/GUS gene is similar to the distribution pattern of ^<14>C-IAA that is applied at the apical end of the gravistimulated shoot. Thus, the results reported here provide additional support for a crucial role of auxin in shoot gravi- and photo-tropisms and adventitious root formation. Furthermore, the data presented in this report also indicate that expression of the GH3/GUS gene is an excellent molecular marker for studying auxin transport and for monitoring changes in either auxin concentration or cellular sensitivity to auxin in planta.
- 日本植物生理学会の論文
著者
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Wu Yan
Department Of Anatomy Tongji Medical College Huazhong University Of Science And Technology
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Li Yi
Department Of Plant Science
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Li Yi
Department Of Applied Chemistry Graduate School Of Engineering Tokyo Metropolitan University
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Hagen Gretchen
Department of Biochemistry, University of Missouri
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Guilfoyle Tom
Department of Biochemistry, University of Missouri
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Guilfoyle Tom
Department Of Biochemistry University Of Missouri
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Hagen Gretchen
Department Of Biochemistry University Of Missouri
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