Integrin-Like Proteins are Localized to Plasma Membrane Fractions, not Plastids, in Arabidopsis

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概要

• 論文の詳細を見る

• Integrins are a large family of integral membrane proteins that function in signal transduction in animal systems. These proteins are conserved in vertebrates, invertebrates, and fungi. Evidence from previous research suggests that integrin-like proteins may be present in plants as well, and that these proteins may function in signal transduction during gravitropism. In past studies, researchers have used monoclonal and polyclonal antibodies to localize β_1 integrin-like proteins in plants. However, there is a disparity between data collected from these studies, especially since molecular weights obtained from these investigations range from 55-120 kDa for integrin-like proteins. To date, a complete investigation which employs all three basic immunolabeling procedures, immunoblotting, immunofluorescence microscopy, and immunogold labeling, in addition to extensive fractionation and exhaustive controls, has been lacking. In this paper, we demonstrate that use of a polyclonal antibody against the cytoplasmic domain of avian β_1-integrin can produce potential artifacts in immunolocalization studeis. However, these problems can be eliminated through use of starchless mutants or proper specimen preparation prior to electrophoresis. We also show that this antibody, when applied within the described parameterts and with careful controls, identifies a large (100 kDa) integrin-like protein that is localized to plasma membrane fractions in Arabidopsis.

• 日本植物生理学会の論文

著者

• Kiss John

  Department Of Botany Miami University

• Edelmann R

  Department Of Botany Miami University

• Edelmann Richard

  Department Of Botany Miami University

• Swatzell Lucinda

  Department of Botany, Miami University

• Makaroff Christopher

  Department of Chemistry and Biochemistry, Miami University

• Swatzell Lucinda

  Department Of Botany Miami University

• Makaroff Christopher

  Department Of Chemistry And Biochemistry Miami University

関連論文

• Reduced Gravitropism in Hypocotyls of Starch-Deficient Mutants of Arabidopsis
• Curvature in Arabidopsis Inflorescence Stems Is Limited to the Region of Amyloplast Displacement
• The Roles of Phytochromes in Elongation and Gravitropism of Roots
• The Development of Spaceflight Experiments with Arabidopsis as a Model System in Gravitropism Studies
• Integrin-Like Proteins are Localized to Plasma Membrane Fractions, not Plastids, in Arabidopsis
• Phototropism and Gravitropism in Lateral Roots of Arabidopsis

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