Identification of a Novel Triterpenoid Saponin from Pisum sativum as a Specific Inhibitor of the Diguanylate Cyclase of Acetobacter xylinum

スポンサーリンク

概要

• 論文の詳細を見る

• A specific and highly potent inhibitor of diguanylate cyclase, the key regulatory enzyme of the cellulose synthesizing apparatus in the bacterium Acetobacter xylinum, was isolated from extracts of etiolated pea shoots (Pisum sativum). The inhibitor has been purified by a multistep procedure, and sufficient amounts of highly purified compound (3-8 mg) for spectral analysis were obtained. The structure of this compound was established as 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranosyl soyasapogenol B 22-O-α-D-glucopyranoside. The structure was elucidated on the basis of susceptibility to various enzymes, chemical and spectral methods, such as GC-MS, FAB-MS, and the following types of 2D-NMR: COSY, ROESY, TOCSEY, HMQC, HMBC analyses. An identical or a very similar compound with identical biological activity was also isolated from A.xylinum, strongly suggesting that at least certain aspects of cellulose synthesis in the bacteria and in higher plants may be regulated in a similar manner. The content of this saponin in etiolated plants was about 0.04 μmol (g fresh tissue)^<-1>.

• 日本植物生理学会の論文

著者

• Delmer D

  Univ. California Ca Usa

• Delmer Deborah

  Section Of Plant Biology University Of California

• Ohana Patricia

  Department of Biological Chemistry, Institute of Life Scineces, The Hebrew University of Jerusalem

• Benziman Moshe

  Department of Biological Chemistry, Institute of Life Scineces, The Hebrew University of Jerusalem

• Carlson Russell

  Biochemistry Department, Complex Carbohydrate Research Center, Life Sciences Building, University of

• Glushka John

  Biochemistry Department, Complex Carbohydrate Research Center, Life Sciences Building, University of

• Azadi Parastoo

  Biochemistry Department, Complex Carbohydrate Research Center, Life Sciences Building, University of

• Bacic Tony

  Plant Cell Biology Research Center, School of Botany, The University of Melbourne

• Benziman Moshe

  Department Of Biological Chemistry Institute Of Life Sciences The Hebrew University

• Carlson Russell

  Biochemistry Department Complex Carbohydrate Research Center Life Sciences Building University Of Ge

• Azadi Parastoo

  Biochemistry Department Complex Carbohydrate Research Center Life Sciences Building University Of Ge

• Bacic Tony

  Plant Cell Biology Research Center School Of Botany The University Of Melbourne

• Glushka John

  Biochemistry Department Complex Carbohydrate Research Center Life Sciences Building University Of Ge

• Ohana Patricia

  Department Of Biological Chemistry Institute Of Life Sciences The Hebrew University

関連論文

• Glycosylated Triterpenoid Saponin : a Specific Inhibitor of Diguanylate Cyclase from Acetobacter xylinum.Biological Activity and Distribution
• Identification of a Novel Triterpenoid Saponin from Pisum sativum as a Specific Inhibitor of the Diguanylate Cyclase of Acetobacter xylinum
• セルロース合成の分野での最近の進歩

スポンサーリンク