Isolation and Characterization of a Water Stress-Specific Genomic Gene, pwsi 18, from Rice

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概要

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• One of the water stress-specific cDNA clones of rice characterised previously, wsi18, was selected for further study. The wsi18 gene can be induced by water stress conditions such as mannitol, NaCl, and dryness, but not by ABA, cold, or heat. A genomic clone for wsi18, pwsi18, contained about 1.7 kbp of the 5' upstream sequence, two introns, and the full coding sequence. The 5'-upstream sequence of pwsi18 contained putative cis-acting elements, namely an ABA-responsive element (ABRE), three G-boxes, three E-boxes, a MEF-2 sequence, four direct and two inverted repeats, and four sequences similar to DRE, which is involved in the dehydration response of Arabidopsis genes. The gusA reporter gene under the control of the pwsi18 promoter showed transient expression in response to water stress. Deletion of the downstream DRE-like sequence between the distal G-boxes-2 and -3 resulted in rather low GUS expression.

• 日本植物生理学会の論文

著者

• KISAKA Hiroaki

  Institute of Genetic Ecology, Tohoku University

• Kisaka Hiroaki

  Institute Of Genetic Ecology Tohoku University

• Kitagawa Yoshichika

  Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul

• Joshee Nirmal

  Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agric

• Joshee Nirmal

  Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul

関連論文

• Fertile Transgenic Asparagus Plants Produced by Agrobacterium-mediated Transformation
• The Role of Aquaporin RWC3 in Drought Avoidance in Rice
• Isolation and Characterization of a Water Stress-Specific Genomic Gene, pwsi 18, from Rice
• Transformation of Soybean by Infecting Embryonic Calli with Agrobacterium tumefaciens and That of Soybean and Kidney Bean by Injecting the Bacteria into Germinating Seeds.

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