Characterisation of Acidic and Basic Apoplastic Peroxidases from Needles of Norway Spruce (Picea abies, L., Karsten) with Respect to Lignifying Substrates

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概要

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• Acidic and basic peroxidases, termed as POD-A and POD-B, were isolated from the apoplastic space of spruce (Picea abies, L.) needles and purified by acetone precipitation and anion exchange chromatography to apparent homogeneity. The molecular masses of POD-A and POD-B were 39.6 and 29.0 kDa, respectively. The pH optimum of both isozymes ranged from 4.5 to 6. The apparent K_m values of POD-A and POD-B were 460 and 210 μM for coniferyl alcohol. Both isozymes acted also as NADH oxidases with apparent K_m-values of 103 μM (POD-A) and 70 μM (POD-B). NAD^+ but not NADH was found in the apoplastic space of lignifying needles. Based on the lignification rate, the contents and kinetic properties of PODs, NADH oxidation by POD is not the major source of H_2O_2 required for lignin polymerisation.

• 日本植物生理学会の論文

著者

• Polle Andrea

  Georg-august University Of Gottingen Institut Fur Forstbotanik Abteilung I:forstbotanik Und Baumphys

• Otter Tilman

  Albert-ludwigs-universitat Institut Fur Forstbotanik Und Baumphysiologie

関連論文

• The Influence of Apoplastic Ascorbate on the Activities of Cell Wall-Associated Peroxidase and NADH Oxidase in Needles of Norway Spruce(Picea abies L.) : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
• Characterisation of Acidic and Basic Apoplastic Peroxidases from Needles of Norway Spruce (Picea abies, L., Karsten) with Respect to Lignifying Substrates

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