Pectin Depolymerase Activities Associated with Cell Walls from Cicer arietinum L. Epicotyl
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概要
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The hydrolytic activity of the proteins extracted with 3 M LiCI from chick-pea (Cicer arietinum) cell walls to pectic fractions extracted with 50 mM trans-1,2-diaminocy-clohexane-.N,N,N′,N′,-tetraacetic acid (CDTA) and 50 mM sodium carbonate was studied. The pectic fractions contain-ed acidic polysaccharides with high molecular mass (higher than 5 x 10^3 kDa), mainly composed of uronic acids, galac-tose, arabinose and rhamnose. The extracted proteins depo-lymerized the pectic polysaccharides and also a commercial preparation of polygalacturonic acid from citrus, detected by a decrease in their viscosity and a shift of their molecular mass distribution. The extract was able to depolymerize a uronic acid-rich component in all the cases, although in different extent. Also, with regard to the CDTA-soluble pectins, a degradation of polyuronide and a shift of the molecular mass distribution of arabinogalactan was observed.
- 日本植物生理学会の論文
著者
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Acebes Jose
Laboratorio De Fisiologia Vegetal Facultad De Biologla Universidad De Leon
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Zarra Ignacio
Laboratorio De Fisiologia Vegetal Facultad De Biologia Universidad De Santiago
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Sevillano Jose
Laboratorio de Fisiologia Vegetal, Facultad de Biologla, Universidad de Leon
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Sevillano Jose
Laboratorio De Fisiologia Vegetal Facultad De Biologla Universidad De Leon
関連論文
- Autolysis Promotes the Extension Capacity of Zea mays Coleoptile Cell Walls in Response to Acid pH Solutions
- Pectin Depolymerase Activities Associated with Cell Walls from Cicer arietinum L. Epicotyl