Effect of GA_3 on the Molecular Mass of Polyuronides in the Cell Walls of Alaska Pea Roots

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概要

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• The role of cell wall matrix polysaccharides in gibberellin-regulated root growth is unknown. We examined pectic polysaccharides from pea roots treated with or with-out gibberellin A_3 (GA_3) in the presence of ancymidol, an inhibitor of gibberellin biosynthesis. Pectic polymers solubilized by CDTA (trans-1,2-cyclohexanediamine-N.N.N'.N'-tetraacetic acid) at 23℃ and subjected to gel permeation analysis exhibited high polydispersity with a molecular mass in excess of 500 kDa. Subsequent extraction of cell walls with CDTA at 100℃ solubilized polymers with an average mol mass of 10 to 40 kDa. Subjecting the high molecular mass pectic polymers extracted at 23℃ to 70-100℃ for 2 h generated 10 to 40 kDa fragments, similar in size distribution to those solubilized directly from cell walls by CDTA solutions at 100℃. Pectic polymers from (GA_3+Anc)-treated roots were of higher average mol mass than those from Anc-treated roots in both the elongation zone and in the basal maturation zone. Since (GA_3+Anc)-treated roots elongate more quickly than Anc-treated roots [Tanimoto (1994) Plant Cell Physiol. 35: 1019], the slender, GA_3-treated roots may produce and deposit highly integrated pectins more rapidly than the thicker. Anc-treated roots in the elongating or elongated cell walls.

• 日本植物生理学会の論文

著者

• Tanimoto Eiichi

  Biological Laboratory College Of General Education Nagoya City University

• Huber Donald

  Biology Laboratory College Of General Education Nagoya City University:(present)horticultural Scienc

関連論文

• Effect of GA_3 on the Molecular Mass of Polyuronides in the Cell Walls of Alaska Pea Roots
• Correlation between β-galactosidase and auxin-induced elongation growth in etiolated pea stems
• Gibberellin Regulation of Root Growth with Change in Galactose Content of Cell Walls in Pisum sativum
• Automated Recording of Lettuce Root Elongation as Affected by Auxin and Acid pH in A New Rhizometer with Minimum Mechanical Contact to Roots

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