Complete Disintegration of the Microtubular Cytoskeleton Precedes its Auxin-Mediated Reconstruction in Postmitotic Maize Root Cells

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概要

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• The inhibitory action of 0.1 μM auxin (IAA) on maize root growth was closely associated with a rapid and complete disintegration of the microtubular (MT) cytoskeleton, as visualized by indirect immunofluorescence of tubulin, throughout the growth region. After 30 min of this treatment, only fluorescent spots were present in root cells, accu-mulating either around nuclei or along cell walls. Six h later, in addition to some background fluorescence, dense but partially oriented oblique or longitudinal arrays of, cortical MTs (CMTs) were found in most growing cells of the root apex. After 24 h of treatment, maize roots had adapted to the auxin, as inferred from the slowly recovering elongation rate and from the reassembly of a dense and well-ordered MT cytoskeleton which showed only slight deviations from that of the control root cells. Taxol pre-treatment (100 μM, 24 h) prevented not only the rapid aux-in-mediated disintegration of the MT cytoskeleton but also a reorientation of the CMT arrays, from transversal to longitudinal. The only tissue to show MTs in their cells throughout the auxin treatment was the epidermis. Significant resistance of transverse CMT arrays in these cells towards auxin was confirmed using a higher auxin concentration (100 μM, 24 h). The latter auxin dose also revealed inter-tissue-specific responses to auxin: outer cortical cell files reoriented their CMTs from the transversal to longitudinal orientation, whereas inner cortical cell files lost their MTs. This high auxin-mediated response, associated with the swelling of root apices, was abolished with the pretreatment of maize root with taxol.

• 日本植物生理学会の論文

著者

• Volkmann D.

  Botanisches Institut Rheinische Friedrich-wilhelms-universitat Bonn Department Of Plant Cell Biology

• Baluska F.

  Botanisches Institut Rheinische Friedrich-wilhelms-universitat Bonn Department Of Plant Cell Biology

• Barlow P.

  IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol

• Barlow P.

  Iacr-long Ashton Research Station Department Of Agricultural Sciences University Of Bristol

関連論文

• Mastoparan Alters Subcellular Distribution of Profilin and Remodels F-Actin Cytoskeleton in Cells of Maize Root Apices
• Effects of Myosin ATPase Inhibitor 2, 3-Butanedione 2-Monoxime on Distributions of Myosins, F-Actin, Microtubules, and Cortical Endoplasmic Reticulum in Maize Root Apices
• Specific Localizaiton of Arabinogalactan-Protein Epitopes at the Surface of Maize Root Hairs
• Complete Disintegration of the Microtubular Cytoskeleton Precedes its Auxin-Mediated Reconstruction in Postmitotic Maize Root Cells

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