Isolation of a cDNA for a Phosphoenolpyruvate Carboxylase from a Monocot CAM-Plant, Aloe arborescens :Structure and its Gene Expression

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概要

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• A phosphoenolpyruvate carboxylase (PEPCase) cDNA was isolated from Aloe arborescens, a monocot CAM plant. Northern analysis of the PEPCase transcript indicat-ed that it is specifically expressed in green leaves, strongly suggesting its involvement in CAM photosynthesis. No diurnal change in expression level was evident. Western blot analysis also showed no alteration of the amount of the PEPCase protein. These results suggest that circadian rhythm in PEPCase activity may be regulated post-transla-tionally. The representative cDNA clone contained an ORF encoding 964 amino acid residues. Deduced amino acid sequence of the aloe PEPCase is highly conserved as compared with other PEPCases. The phosphorylation site which may be modified by PEPC-kinase was conserved. An evolutional map with known PEPCases suggested that CAM-type PEPCases were located between C4 and house-keeping PEPCases.

• 日本植物生理学会の論文

著者

• Honda Hideo

  Life Science Institute Mitsui Toatsu Chemicals Inc.

• Shimada Hiroaki

  Life Science Institute Mitsui Toatsu Chemicals Inc.:(present)department Of Biological Science And Te

• Okamoto Tomoko

  Life Science Institute Mitsui Toatsu Chemicals Inc.

関連論文

• Isolation of a cDNA for a Phosphoenolpyruvate Carboxylase from a Monocot CAM-Plant, Aloe arborescens :Structure and its Gene Expression

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