Tomato (Lycopersicon esculentum cv. Pik-Red) Leaf Carboxypeptidase: Identification, N-terminal Sequence, Stress-Regulation, and Specific Localization in the Paraveinal Mesophyll Vacuoles

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概要

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• Wounding of tomato (Lycopersicon esculentum L.) leaves causes systemic induction of a serine-type carboxypeptidase activity. We find this activity to be present in several isoforms. Antibodies raised against the leaf carboxypeptidase inhibited the enzyme activity and the immuno-precipitates were resolved into a 69-kDa polypeptide and a doublet of 35/37-kDa proteins on SDS-PAGE. Immuno-blot analysis of the leaf proteins also immunodecorated the 69-kDa and 35/37-kDa proteins. Amino acid sequence analysis of the amino-terminus of the tomato leaf 69-kDa carboxypeptidase showed it to be similar to the barley A-chain carboxypeptidase I [Sorenson et al. (1986) Carlsberg Res. Commun. 51: 475], sharing Ala as the N-terminus and the sequences, AlaProGln and LeuProGlyPhe. Superimposition of a chemical stress (copper treatment) on wounding apparently lowered wound-induced carboxypeptidase activity in the leaf, suggesting that cupric ions might interact with the wound signal. Immunogold electron microscopy indicated that the leaf carboxypeptidase was specifically localized within the inclusions of vacuoles of vascular parenchyma cells. In cupric ion-treated tissues, carboxypeptidase was found redistributed to other parts of the cell, indicating that this treatment, but not wounding, causes general vacuolar membrane damage.

• 日本植物生理学会の論文

著者

• Mattoo Autar

  Plant Molecular Biology Laboratory Beltsville Agricultural Research Center

• Mattoo Autar

  Plant Moleclar Biology Laboratory

• Mehta Roshni

  Plant Molecular Biology Laboratory Beltsville Agricultural Research Center:department Of Bioiogical

• Warmbardt Robert

  Plant photobiology Laboratory, Beltsville Agricultural Research Center

• Warmbardt Robert

  Plant Photobiology Laboratory Beltsville Agricultural Research Center

関連論文

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• Differential Protein Metabolism and Gene Expression in Tomato Fruit during Wounding Stress
• Tomato (Lycopersicon esculentum cv. Pik-Red) Leaf Carboxypeptidase: Identification, N-terminal Sequence, Stress-Regulation, and Specific Localization in the Paraveinal Mesophyll Vacuoles
• A Wound-Repressible Glycine-Rich Protein Transcript is Enriched in Vascular Bundles of Tomaoto Fruit and Stem : PROTEINS, ENZYMES AND METABOLISM

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