Two Plasma Membrane H^+_ATPase Genes Expressed in Guard Cells of Vicia faba Are Also Expressed Throughout the Plant

スポンサーリンク

概要

• 論文の詳細を見る

• Guard cells modulate stomatal apertures in response to hormones, metabolic demands and environmental stimuli. The guard cell PM H^+-ATPases play a critical role in this process by generating the electrochemical gradient to drive solute transport and concomitant water flux. The PM H^+-ATPase activity is specifically regulated by red and blue light, fungal toxins and auxin. To determine if the unique responsiveness of the guard cell PM H^+-ATPase is due to the expression of a cell-specific isoform, we amplified by PCR, and cloned portions of PM H^+-ATPase genes VHA1 and VHA2, which are expressed in guard cell protoplasts (GCP). In situ hybridization to leaf tissue sections indicated that VHA1 and VHA2 genes were expressed in guard cells and mesophyll cells but not in epidermal cells or vascular tissues. Furthermore, a gene-specific quantitative reverse transcription (RT)-PCR detected VHA1 and VHA2 mRNAs in both GCP and mesophyll cell protoplast mRNA as well as in mRNA isolated from roots, leaves, stems and flowers. Thus, two PM H^+-ATPase genes ex-pressed in guard cells are also expressed in many other tissues and cell types. This suggests that the unique responsiveness of the guard cell PM H^+-ATPases to environmental stimuli results from cell-specific signal transduction pathways rather than the expression of a cell-specific PM H^+-ATPase.

• 日本植物生理学会の論文

著者

• Schroeder Julian

  Department Of Biology University Of California

• Schroeder Julian

  Division Of Biology Cell And Developmental Biology Section And Center For Molecular Genetics Univers

• Hentzen Audrey

  Mann Laboratory, Department of Vegetable Crops, University of California

• Smart Lawrence

  Mann Laboratory, Department of Vegetable Crops, University of California

• Wimmers Larry

  Mann Laboratory, Department of Vegetable Crops, University of California

• Fang Henry

  Department of Biology, University of California

• Bennett Alan

  Mann Laboratory, Department of Vegetable Crops, University of California

• Fang Henry

  Department Of Biology University Of California

• Bennett Alan

  Mann Laboratory Department Of Vegetable Crops University Of California

• Wimmers Larry

  Mann Laboratory Department Of Vegetable Crops University Of California:(present)department Of Biolog

• Smart L

  State Univ. New York Coll. Environmental Sci. And Forestry Ny Usa

• Hentzen Audrey

  Mann Laboratory Department Of Vegetable Crops University Of California:(present)school Of Health And

関連論文

• Two Plasma Membrane H^+_ATPase Genes Expressed in Guard Cells of Vicia faba Are Also Expressed Throughout the Plant
• Overexpression of Phytochelatin Synthase in Arabidopsis Leads to Enhanced Arsenic Tolerance and Cadmium Hypersensitivity
• 3Ap04 TRANSGENIC EXPRESSION OF TOMATO POLYGALACTU RONASE IN TOBACCO AND MUTANT TOMATO GENOTYPES

スポンサーリンク