Developmental and Tissue-Specific Expression of JIP-23, a Jasmonate-Inducible Protein of Barley

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概要

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• Developmental expression of a 23 kDa jasmonate-in-duced protein (JIP-23) of barley leaves (Hordeum vulgare cv. Salome) was studied by measuring the time-dependent accumulation of transcript and protein during germination. Tissue-specific expression of JIP-23 was analyzed immunocytochemically and by in situ hybridizations, respectively. During seed germination JIP-23 mRNA was found to accumulate transiently with a maximum at 32 h, whereas the protein was steadily detectable after the onset of expression. The occurrence of new isoforms of JIP-23 during germination in comparison to jasmonate-treated leaves suggests, that the JIP-23 gene family of barley is able to express different subsets of isoforms dependent on the developmental stage. JIP-23 and its transcript were found mainly in the scutellum, the scutellar nodule and in lower parts of the primary leaf of 6 days old seedlings. All these tissues exhibited high levels of endogenous jasmonates. In situ hybridization revealed specific accumulation of JIP-23 mRNA in companion cells of the phloem in the nodule plate of the scutellum. In accordance with that, JIP-23 was detected im-munocytochemically in phloem cells of the root as well as of the scutellar nodule and in parenchymatic cells of the scutellum. The cell type-specific occurrence of JIP-23 was restricted to cells, which are known to be highly stressed osmotically by active solute transport. This observation suggests, that the expression of this protein might be a response to osmotic stress during development.

• 日本植物生理学会の論文

著者

• HAUSE Bettina

  Department of Secondary Metabolism, Leibniz Institute of Plant Biochemistry

• WASTERNACK Claus

  Department of Natural Product Biotechnology, Leibniz Institute of Plant Biochemistry

• Hause B

  Department Of Secondary Metabolism Leibniz Institute Of Plant Biochemistry

• Hause Bettina

  Institut Fur Pflanzenbiochemie

• Wasternack Claus

  Department Of Natural Product Biotechnology Leibniz Institute Of Plant Biochemistry

• Demus Uta

  Institute of Plant Biochemistry Halle

• Teichnrann Christiane

  Institute of Plant Biochemistry Halle

• Parthier Benno

  Institute of Plant Biochemistry Halle

• Wasternack Claus

  Institute of Plant Biochemistry Halle

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