Molecular Cloning and Characterization of the cDNA for Discoidin II of Dictyostelium discoideum

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概要

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• By using monoclonal antibodies directed against dis-coidin II, we have isolated clones from axenically grown Ax-2 cells. One clone (D2) contained a 1.2-kb insert encoding the entire discoidin II protein, which is composed of 257 amino acid residues and has a calculated molecular mass of 28,574. The amino acid sequences, deter-mined by Edman degradation of six tryptic peptides of discoidin II, were identical to those deduced from the sequence. The protein bears no resemblance to any proteins in the data banks, except that its sequence is 49% identical with the amino acid sequence of discoidin I. Discoidin II shares with discoidin I both a carbohydrate-binding site and an Arg-Gly-Asp (RGD) sequence, which has been found in fibronectin in mammalian cells. With the onset of aggregation (8 h of development), a 1.3-kb discoidin II mRNA begins to accumulate. A similar pattern of regulation occurs at the protein level.

• 日本植物生理学会の論文

著者

• Fukuzawa Masashi

  Division Of Biological Sciences Graduate School Of Science Hokkaido University:(present)mrc Laborato

• Ochiai Hiroshi

  Division Of Biological Science Graduate School Of Science Hokkaido University

関連論文

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• Inhibitory Effects of Tunicamycin on Cell-Cell Adhesion and Cell Reaggregation of the Cellular Slime Mold, Polysphondylium pallidum
• Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum development
• A novel Dictyostelium Cdk8 is required for aggregation, but is dispensable for growth
• Molecular Cloning and Characterization of the cDNA for Discoidin II of Dictyostelium discoideum

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