Two Polypeptides Inducible by Low Levels of CO_2 in Soluble Protein Fractions from Chlorella regularis Grown at Low or High pH
スポンサーリンク
概要
- 論文の詳細を見る
Previous studies suggested that certain protein(s) other than carbonic anhydrase might play an important role in the facilitated transport of dissolved inorganic carbon (DIC) from the medium to the site of CO_2 fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase in the unicellular green alga Chlorella regularis adapted to low-CO_2 (ordinary air) conditions [Shiraiwa et al. (1991) Jpn. J. Phycol. 39: 355; Satoh and Shiraiwa (1992) Research in Photosynthesis, Vol. III, p. 779]. The proteins that might be involved in this facilitated transport of DIC were investigated by pulse-labeling of induced proteins with ^<35>S-sulfate during adaptation of cells grown under high-CO_2 conditions to low CO_2. Analysis by SDS-PAGE revealed that synthesis of two polypeptides, with molecular masses of 98 and 24 kDa, respectively, was induced under low-CO_2 con-ditions. The 24-kDa polypeptide was induced at pH 5.5 but not at pH 8.0, whereas the 98-kDa polypeptide was induced at both pH 5.5 and pH 8.0. The possible role of these polypeptides in the facilitated transport of DIC in Chlorella regularis is discussed.
- 日本植物生理学会の論文
著者
-
Shiraiwa Yoshihiro
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Shiraiwa Yoshihiro
Graduate School Of Science And Technology Niigata University:department Of Biology Faculty Of Scienc
-
Satoh Akira
Graduate School of Science and Technology, Niigata University
-
Satoh Akira
Graduate School Of Science And Technology Niigata University
-
Shiraiwa Yoshihiro
Graduate School Of Life And Environmental Sciences
関連論文
- Change of the unsaturation degree of alkenone and alkenoate during acclimation to salinity change in Emiliania huxleyi and Gephyrocapsa oceanica with reference to palaeosalinity indicator
- A Synechocystis Homolog of SipA Protein, Ssl3451, Enhances the Activity of the Histidine Kinase Hik33
- Induction of a High-CO_2-Inducible, Periplasmic Protein, H43, and its Application as a High-CO_2-Responsive Marker for Study of the High-CO_2-Sensing Mechanism in Chlamydomonas reinhardtii
- Photosynthetic Carbon Assimilation in the Coccolithophorid Emiliania huxleyi (Haptophyta) : Evidence for the Predominant Operation of the C_3 Cycle and the Contribution of β-Carboxylases to the Active Anaplerotic Reaction
- Pyruvate carboxylase is involved in photosynthetic carbon fixation by the coccolithophorid, Emiliania huxleyi
- Physiological Regulation of Coccolith Polysaccharide Production by Phosphate Availability in the Coccolithophorid Emiliania huxleyi
- Two Polypeptides Inducible by Low Levels of CO_2 in Soluble Protein Fractions from Chlorella regularis Grown at Low or High pH
- Compensatory response of the unicellular-calcifying alga Emiliania huxleyi (Coccolithophoridales, Haptophyta) to ocean acidification
- Proteomic Analysis of High-CO_2-Inducible Extracellular Proteins in the Unicellular Green Alga, Chlamydomonas reinhardtii
- Enzymatic Inactivation of Extracellular Carbonic Anhydrase and its Effect on K_ (CO_2) for Photosynthesis in Chlorella ellipsoidea C-27
- Low Temperature Stimulates Cell Enlargement and Intracellular Calcification of Coccolithophorids
- Characterization of the Selenite Uptake Mechanism in the Coccolithophore Emiliania huxleyi (Haptophyta)
- Technical improvement in the purification of enzymes from red algae using an aqueous two-phase partitioning system
- Characterization of the Subdomains in the N-Terminal Region of Histidine Kinase Hik33 in the Cyanobacterium Synechocystis sp. PCC 6803
- Enzymological Evidence for the Function of a Plastid-Located Pyruvate Carboxylase in the Haptophyte alga Emiliania huxleyi : A Novel Pathway for the Production of C_4 Compounds
- Changes in alkenone and alkenoate distributions during acclimatization to salinity change in Isochrysis galbana : Implication for alkenone-based paleosalinity and paleothermometry