Microelectrophoretic and 9-Aminoacridine Fluorescence Study of the Surface Electrical Properties of Suspension Cultured Catharanthus roseus Cells and Isolated Protoplasts : Effects of Abscisic Acid Treatment : MEMBRANES AND BIOENERGETICS

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The effects of abscisic acid (ABA) treatments on the surface electrical properties of cells and isolated protoplasts from Catharanthus roseus cell suspension cultures were studied by electrophoretic mobility and 9-aminoacridine (9AA) fluorescence measurements. The surface charge densities of the cells and protoplasts estimated from electrokinetic data were -0.064 C m^<-2> and - 0.048 C m^<-2> respectively. These values were close to that estimated by 9AA fluorescence technique i.e., -0.053 C m^<-2> for the cells and -0.041 C m^<-2> for the isolated protoplasts accordingly. The net negative surface charge density decreased after application of 10 μM and 50 μM ABA in both cells and protoplats, the more pronounced effect being observed at 10 μM ABA. When 100 μM ABA was supplemented to the cell suspension culture the opposite effect was observed. The average charge density increased to -0.074 C m^<-2> for the cells, and to -0.055 C m^<-2> for protoplasts, as revealed from the 9AA measurements. The results are discussed in terms of specific concentration dependent ABA-induced alterations of the electrostatic properties of cell and protoplast membranes.
日本植物生理学会の論文