Enzymatic Conversion of Volatile Metabolites in Dry Seeds during Storage : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM

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概要

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• Dry seeds can transform volatile metabolites via enzymes pre-existing in them. The interconversion between acetaldehyde and ethanol occurred when they were applied gaseously to seeds of rice, lettuce, pea, cocklebur during storage at different relative humidities (RH) at either 23℃ or -3.5℃. Interconversions between the compounds decreased with decreasing RH from 75% to 12% regardless of seed species and storage temperature, but it was still detectable even at 12% RH at -3.5℃. However, the conversion from acetaldehyde to ethanol did not occur when seeds were killed by heating prior to storage. 2-Methoxyethanol, a competitive inhibitor of alcohol dehydrogenase (ADH) (EC 1.1.1.1), suppressed the transformation from ethanol to acetaldehyde in lettuce seeds. Therefore, ADH pre-existing in dry seeds may be involved in the interconversion between ethanol and acetaldehyde in dry seeds. Propanal, also a substrate of ADH, could be transformed to propanol during storage. However, methanol, which is not a substrate of this enzyme, was hardly converted to formaldehyde. Ethylacetate, applied to seeds during storage, was hydrolyzed by both lettuce and rice seeds, and the amount of hydrolysis increased with increasing RH. Similarly, other carboxylic esters, such as methylacetate, ethylformate and ethylpropionate, could be hydrolyzed by rice, pea, and lettuce seeds. Little hydrolysis of ethylacetate occurred in the seeds killed by heating before storage, suggesting that ethylacetate may be hydrolyzed by carboxylic-ester hydrolase (EC 3.1.1.1). On the other hand, the production of ethylacetate through esterification was demonstrated by addition of gaseous acetate and ethanol in lettuce seeds, which occurred more greatly at 12% RH than at 75% RH. These findings were discussed in relation to the mechanism of seed aging.

• 日本植物生理学会の論文

著者

• Zhang Ming

  Botanical Garden Faculty Of Science North Campus Of Tohoku University

• NAKAMARU Yasuo

  Botanical Garden, Faculty of Science, North Campus of Tohoku University

• TSUDA Sachiko

  Botanical Garden, Faculty of Science, North Campus of Tohoku University

• NAGASHIMA Tazuko

  Botanical Garden, Faculty of Science, North Campus of Tohoku University

• ESASHI Yohji

  Botanical Garden, Faculty of Science, North Campus of Tohoku University

• Esashi Y

  Japan Wood Seed Res. Inst. Ichihara Jpn

• Tsuda Sachiko

  Institute For Advanced Research Suntory Ltd.

• Nakamaru Yasuo

  Botanical Garden Faculty Of Science North Campus Of Tohoku University

• Nagashima Tazuko

  Botanical Garden Faculty Of Science North Campus Of Tohoku University

関連論文

• Aging of Soybean Seeds in Relation to Metabolism at Different Relative Humidities : PROTEINS, ENZYMES AND METABOLISM
• Enzymatic Conversion of Volatile Metabolites in Dry Seeds during Storage : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
• P-158 The Nature of Normal Faults under Variable Boundary Conditions : Results from Sandbox Modeling
• Promotion of Cocklebur Seed Germination by Allyl, Sulfur and CyanogenicCompounds
• Involvement of Cyanogens in the Promotion of Germination of Cocklebur Seeds in Response to Various Nitrogenous Compounds, Inhibitors of Respiratory and Ethylene
• Further Study on the Involvement of Cyanide-Resistant Respiration in the Germination of Cocklebur Seeds
• Interrelation between Ethylene and Carbon Dioxide in Relation to Respiration and Adenylate Content in the Pre-Germination Period of Cocklebur Seeds
• Changes in Ethylene Production and in 1-Aminocyclopropane-1-carboxylic Acid (ACC) and Malonyl-ACC Contents of Cocklebur Seeds during Their Development

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