Isolation and Characterization of the Plasma Membrane by Two-Phase Partitioning from the Alkalophilic Cyanobacterium Spirulina platensis : MEMBRANES AND BIOENERGETICS

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概要

• 論文の詳細を見る

• Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3-4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg^<2+>-ATPase activity that is sensitive to vanadate, stimulated by K^+, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.

• 日本植物生理学会の論文

著者

• Boussiba Sammy

  Microalgal Biotechnology Laboratory, The Jacob Blaustein Institute for Desert Research

• Boussiba Sammy

  Microalgal Biotechnology Laboratory The Jacob Blaustein Institute For Desert Research Ben-gurion Uni

• Boussiba Sammy

  Microalgal Biotechnology Laboratory Albert Katz Department Of Dryland Biotechnologies Jacob Blaustei

• XU Chang

  Microalgal Biotechnology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion U

• NEJIDAT Ali

  Environmental Microbiology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion

• BELKlN Shimshon

  Environmental Microbiology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion

• Xu Chang

  Microalgal Biotechnology Laboratory The Jacob Blaustein Institute For Desert Research Ben-gurion Uni

• Nejidat Ali

  Environmental Microbiology Laboratory The Jacob Blaustein Institute For Desert Research Ben-gurion U

• Belkln Shimshon

  Environmental Microbiology Laboratory The Jacob Blaustein Institute For Desert Research Ben-gurion U

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• Isolation and Characterization of the Plasma Membrane by Two-Phase Partitioning from the Alkalophilic Cyanobacterium Spirulina platensis : MEMBRANES AND BIOENERGETICS
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