Synthesis of Cell Wall Galactans from Flax(Linum usitatissimum L.)Suspension-Cultured Cells : PROTEINS, ENZYMES AND METABOLISM

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Cell walls of flax suspension-cultured cells contain both β1→4 galactan (most of which can be extracted with boiling water) and β1→3, β1→6 galactan (most of which can be solubilized with alkali). The relative level of β1→4 galactan in the cell walls is maximal during the lag phase, accounting for an average of 8% of the dry cell-wall mass, whereas the relative level of β1→3, β1→6 galactan increases throughout growth, reaching a maximum (up to 20%) during the exponential phase. Two peaks of galactan synthase activity can be detected that catalyze the synthesis of β1→4 galactan at pH 8 during the lag phase and the synthesis of β1→3, β1→6 galactan at pH 5 during the growth phase. At the end of the growth phase both activities are negligible.
日本植物生理学会の論文