Endo-I,4-β-Glucanase in the Cell Wall of Stems of Auxin-Treated Pea Seedlings : PROTEINS, ENZYMES AND METABOLISM

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概要

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• Endo-1,4-β-glucanase induced by treatment of pea seedlings with 2,4-D was extracted from a preparation of the walls of epicotyl cells. The β-glucanase was purified by chromatography on DEAE-cellulose, affinity chromatography on Con A-Sepharose and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The activity of β-glucanase was retained after removal of SDS and extraction from polyacrylamide gels. The band of a protein (46 kDa), that corresponded to the activity of endo-1,4-β-glucanase, was injected directly into mice for preparation of antiserum and the protein was also subjected to amino acid sequencing after blotting onto a membrane. Western blot analysis showed that the antiserum obtained bound to a 46-kDa polypeptide and recognized endo-1 ,4-β-glucanase. The N-terminal sequence of the 46-kDa polypeptide revealed some homology to abscission endo-1,4-β-glucanases of bean and avocado fruit.

• 日本植物生理学会の論文

著者

• Hayashi Takahisa

  Basic Research Laboratory Central Research Laboratories Ajinomoto Co. Ltd.

• Ohsumi Chieko

  Basic Research Laboratory, Central Research Laboratories, Ajinomoto Co., Inc.

• Ohsumi Chieko

  Basic Research Laboratories Ajinomoto Co. Inc.

• Ohsumi Chieko

  Basic Research Laboratory Ajinomoto Co. Inc.

• Hayashi Takahisa

  Basic Research Laboratory Ajinomoto Co. Inc.:(current)wood Research Institute Kyoto University

関連論文

• Gas Chromatographic Analysis of Alliin in the Callus Tissues of Allium sativum
• Endo-I,4-β-Glucanase in the Cell Wall of Stems of Auxin-Treated Pea Seedlings : PROTEINS, ENZYMES AND METABOLISM
• Effects of Amino Acid Medium on Cell Aggregation in Suspension-cultured Rice Cells
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• The Oligosaccharide Units of the Xyloglucans in the Cell Walls of Bulbs of Onion, Garlic and their Hybrid : STRUCTURE AND FUNCTION OF CELLS

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