Characterization of the Promoter of Rice Plastid psaA-psaB-rps14 Operon and the DNA-Specific Binding Proteins

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概要

• 論文の詳細を見る

• Transcriptional analysis of the promoter of rice psaA-psaB-rps14 operon was performed with Bal 31 deleted mutants in spinach chloroplast extracts. Two functional promoters in this operon (denoted as " - 175" and " - 129" promoters) were revealed to be utilized by the spinach transcription machinery; however only the " - 175" promoter is utilized in vivo. The existence of psaA promoter-specific binding factors in the chloroplast extracts was indicated by competitive gel retardation assay and exonuclease III protection analysis. After ammonium sulfate fractionation of the extracts followed by heparin-agarose column chromatography, the sequence-specific binding factors were characterized to be 66-kDa and 31-kDa polypeptides. Southwestern analysis demonstrated that the two protein factors interact independently with the psaA promoter-containing DNA fragment.

• 日本植物生理学会の論文

著者

• CHEN Shu-Chen

  Institute of Botany, Academia Sinica

• CHENG Ming-Chih

  Institute of Botany, Academia Sinica

• Chen Shu-chen

  Institute Of Botany Academia Sinica

• Cheng M‐c

  Acad. Sinica Taipei Twn

• CHEN Ming-Chu

  Institute of Biochemistry, National Yang-Ming Medical College

• Cheng Ming-chih

  Institute Of Botany Academia Sinica

関連論文

• Electrophoretic Separation and Characterization of Rice Chlorophyll α-Protein Complexes(Biological Chemistry)
• Characterization of the Promoter of Rice Plastid psaA-psaB-rps14 Operon and the DNA-Specific Binding Proteins
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• Molecular Characterization of a Senescence-Associated Gene Encoding Cysteine Proteinase and its Gene Expression during Leaf Senescence in Sweet Potato
• Characterization of the Light-Responsive Promoter of Rice Chloroplast psbD-C Operon and the Sequence-Specific DNA Binding Factor
• DNA Polymorphisms Generated by Arbitrarily Primed PCR in Rice

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