Enhanced Formation of a Constitutive Sesquiterpenoid in Culturerd Cells of a Liverwort, Calypogeia granulata Inoue during Elicitation : Effects of Vanadate

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The early cellular responses to elicitation by an abiotic elicitor, vanadate, in suspension-cultured cells of the liverwort Calypogeia granulata Inoue were investigated. The rate of production of 1,4-dimethylazulene, which is a constitutive sesquiterpenoid in intact plants and also in cultured cells of C. granulata, was more than double the control rate after treatment with vanadate. This increase was preceded by doubling of the activity of 3-hydroxy-3-methylglutaryl CoA reductase, an enzyme involved in isoprenoid biosynthesis. Activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase increased to 1.5 to 2 times the control activities upon treatment with vanadate. Intracellular levels of glutathione (reduced form) increased to 10 times the control level within 24 h after addition of the elicitor. Furthermore, addition of hydrogen peroxide to the culture also enhanced the production of 1,4-dimethylazulene to the same extent as vanadate. We propose that treatment with the elicitor resulted in oxidative stress due to generation of active oxygen species which might act as messengers to activate the biosynthetic pathway to the formation of 1,4-dimethylazulene on the one hand, and to activation of a system for scavenging active oxygen species on the other.
日本植物生理学会の論文