Proteins Induced by Physical Dtimuli in Root Tips of Arabidopsis thaliana Seedlings

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Proteins newly synthesized in cells of root tips of Arabidopsis seedlings after gravistimulation and photo-induced tactile stimulation were analyzed by two-dimensional gel electrophoresis. Intensities of two out of about 600 protein spots were observed to increase transiently when culture flasks in which seedlings has been grown were kept on their sides. When the flasks were kept vertical on a rocking table and rocked continuously for 24 hours, intensities of ten protein spots increased, and four spots appeared for the first time. Analysis of [^<32>P]-labeled proteins revealed that the continuous rocking treatment enhanced the phosphorylation of proteins in two spots. When the seedlings in flasks were illuminated from the front, and the roots bent towards the back wall of the flasks, three spots appeared for the first time and intensities of nine spots were enhanced. Three of the twelve spots whose intensities were enhanced by the photo-induced tactile stimulation were also affected by continuous rocking treatment. The roles of protein synthesis and phosphorylation in the pathways between the stimuli and the responses are discussed.
日本植物生理学会の論文