Initial and Total Activity of Rubisco in Scenedesmus ecornis : is the French Press a Suitable Tool for Enzyme Extraction?

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概要

• 論文の詳細を見る

• An enzyme assay was developed to measure the initial and CO_2-Mg^;t:2+> activated forms of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the green microalga Scenedesmus ecornis. Comparisons were made of extraction by mortar and pestle versus the French press, in the presence or absence of NADPH. Losses due to harvesting and extraction procedures de-creased when the algae were collected under the same irradiance they had experienced during growth, and by adding NADPH to the extraction solution. Results are discussed in relation to the possible interactions of effectors with the enzyme. The suitability of the French press to obtain catalyiic competent extract is questioned.

• 日本植物生理学会の論文

著者

• Legendre Louis

  Departement De Biologic Universite Laval

• De La

  Groupe De Recherche En Recyclage Biologique Et Aquiculture Universite Laval

• MOUGET Jean-Luc

  Groupe de Recherche en Recyclage Biologique et Aquiculture, Universite Laval

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• Initial and Total Activity of Rubisco in Scenedesmus ecornis : is the French Press a Suitable Tool for Enzyme Extraction?

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