A Novel Method for Detection of Endo-Xyloglucan Transferase : GROWTH AND DEVELOPMENT : PROTEINS, ENZYMES AND METABOLISM
スポンサーリンク
概要
- 論文の詳細を見る
A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.
- 日本植物生理学会の論文
著者
-
Nishitani K
Graduate School Of Science Tohoku Univ.
-
Nishitani Kazuhiko
Department Of Biology Facully Of Science Osaka City University
関連論文
- Carbohydrate-Binding Module of a Rice Endo-β-1,4-glycanase, OsCel9A, Expressed in Auxin-Induced Lateral Root Primordia, is Post-Translationally Truncated
- Genomic Basis for Cell-Wall Diversity in Plants : A Comparative Approach to Gene Families in Rice and Arabidopsis
- A Proteomic Approach to Apoplastic Proteins Involved in Cell Wall Regeneration in Protoplasts of Arabidopsis Suspension-cultured Cells
- Two Azuki Bean XTH Genes, VaXTH1 and VaXTH2, with Similar Tissue-Specific Expression Profiles, are Differently Regulated by Auxin
- A Comprehensive Expression Analysis of all Members of a Gene Family Encoding Cell-Wall Enzymes Allowed us to Predict cis-Regulatory Regions Involved in Cell-Wall Construction in Specific Organs of Arabidopsis
- Reverse Genetic Approach to Exploring Genes Responsible for Cell-Wall Dynamics in Supporting Tissues of Arabidopsis thaliana under Microgravity Conditions
- A Study on the Mechanism of Nodding Initiation of the Flower Stalk in a Poppy, Papaver Rhoeas L.
- シロイヌナズナの木部二次細胞壁に関わる遺伝子群の同定と解析
- INVOVEMENT OF ENDO-XYLOGLUCAN TRANSFERASE (EXGT) GENE DURING THE DIFFERENTAL GROWTH OF PER(Pisum sativum L. ) ROOTS
- Endoxyloglucan Transferase cDNA Isolated from Pea Roots and Its Fluctuating Expression in Hydrotropically Responding Roots
- EXPRESSION OF ENDO-XYLOGLUCAN TRANSFERASE (EXGT) GENE DURING THE HYDROTROPIC RESPONSE OF PEA(Pisum sativum L.) ROOTS
- Modifications of cell wall polysaccharides during auxin-induced growth in azuki bean epicotyl segments
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana
- Mechanical load induces upregulation of transcripts for a set of genes implicated in secondary wall formation in the supporting tissue of Arabidopsis thaliana
- Cell Wall-Related Genes Involved in Supporting Tissue Formation and Transcriptional Regulation in Arabidopsis thaliana
- Growth and cell wall changes in azuki bean epicotyls I. : Changes in wall polysaccharides during intact growth
- The XTH Family of Enzymes Involved in Xyloglucan Endotransglucosylation and Endohydrolysis : Current Perspectives and a New Unifying Nomenclature
- Growth and cell wall changes in azuki bean epicotyls II.Changes in wall polysaccharides during auxin-induced growth of excised segments
- Genotypic Variations in Non-Structural Carbohydrate and Cell-Wall Components of the Stem in Rice, Sorghum, and Sugar Vane
- Functional analyses of an Arabidopsis glycine-rich protein
- Announcement of JPR Awards 2007
- Looking toward the future of plant biology and the Journal of Plant Research
- Awards and changes at the Journal of Plant Research
- A Novel Method for Detection of Endo-Xyloglucan Transferase : GROWTH AND DEVELOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Announcement of JPR Awards 2006
- Auxin-induced changes in the molecular weight of hemicellulosic polysaccharides of the Avena coleoptile cell wall
- New Directions to Post-genomic Cell Wall Research
- From the editor-in-chief : Toward a new era for the Journal of Plant Research
- A genome-based approach to study the mechanisms by which cell-wall type is defined and constructed by the collaborative actions of cell-wall-related enzymes
- Auxin-Induced Changes in the Cell Wall Xyloglucans : Effects of Auxin on the Two Different Subfractions of Xyloglucans in the Epicotyl Cell Wall of Vigna angularis