Effects of Inhibitors on the Light-Induced Changes in Electric Potential in Pulvinar Motor Cells of Phaseolus vulgaris L. : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS

スポンサーリンク

概要

• 論文の詳細を見る

• To analyze the mechanism of the light-induced changes in electric potential in motor cells of the pulvinus of Phaseolus vulgaris L., inhibitors were applied to the pulvinus by the xylem perfusion method. The membrane potential was -60 to -80 mV, which indicated that the polarization was less than that of cells of a pulvinus in air. A pulse (30 s) of blue light (BL) induced transient depolarization of the membrane in the motor cells. Red light (RL) caused hyperpolarization of the membrane. The magnitude of BL pulse-induced transient depolarization was greater under the hyperpolarized state caused by the RL. The membrane was depolarized to -30 to -40 mV on perfusion with the respiratory inhibitor NaN_3 (1 mM) and a pulse of BL or irradiation with RL did not cause any change in the potential in the depolarized state. Hyperpolarization of the membrane by RL was inhibited by perfusion with DCMU (50 μM), an inhibitor of electron transport in photosynthesis. However, the magnitude of the depolarization induced by the pulse of BL was not affected. Perfusion with a proton ionophore CCCP (100 μM) depolarized the membrane and no change in the potential was induced by a pulse of BL or by RL in the depolarized state. The extent of the BL pulse-induced depolarization of the membrane was proportional to the magnitude of the membrane potential at the time of which the pulse of BL was applied. It is suggested that the active component of the membrane potential was inhibited by the pulse of BL. The experimental results further support the hypothesis that BL inhibits the activity of the proton ATPase and, thus, causes loss of the electrogenic component of the membrane potential of the pulvinar motor cells.

• 日本植物生理学会の論文

著者

• NISHIZAKI Yuichiro

  Laboratory of Biology, Faculty of Humanities and Sciences, Kobe Gakuin University

• Nishizaki Yuichiro

  Laboratory Of Biology Faculty Of Humanities And Sciences Kobe Gakuin University

• Nishizaki Yuichiro

  Laboratory Of Biology Faculty Of Humanities And Sciences Kobe Gakuen University

関連論文

• Blue-Light-Dependent Osmoregulation in Protoplasts of Phaseolus vulgaris Pulvini
• A Pulse of Blue Light Induces a Transient Increase in Activity of Apoplastic K^+ in Laminar Pulvinus of Phaseolus vulgaris L.
• Vanadate and Dicyclohexylcarbodiimide Inhibit the Blue Light-Induced Depolarization of the Membrane in Pulvinar Motor Cells of Phaseolus : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
• Effects of Inhibitors on the Light-Induced Changes in Electric Potential in Pulvinar Motor Cells of Phaseolus vulgaris L. : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
• Effects of Blue Light on Electrical Potential and Turgor in Pulvinar Motor Cells of Phaseolus

スポンサーリンク