Identity of a Group of 13.5-20 kDa Polypeptides Whose Apparent Abundance Decreases during Plastid Development : GROWTH AND DEVELOPMENT : MEMBRANES AND BIOENERGETICS

概要

論文の詳細を見る
The identity of a group of 13.5-20 kDa polypeptides whose abundance decreases during the greening of intermittent-light grown barley seedlings was investigated. During greening the relative abundance of the 13.5-20 kDa polypeptides was inversely related to that of LHC II, which had led others to suggest a role of these polypeptides in the assembly of the LHC II and/or chloroplast development. The smallest 13.5 kDa polypeptide was identified as histone H4 by N-terminal sequencing of an internal peptide fragment produced by CNBr cleavage. The entire group of 13.5-20 kDa polypeptides was thereafter verified to be nuclear histones by their similar mobility on SDS-PAGE to that of barley histones and immunoreactivity to yeast histone anti-bodies. Their presence results from contamination of plastid preparations by nucleosomes. Our results unequivocally substantiate earlier suggestions of others that polypeptides often found to contaminate immature plastids were of nuclear origin. Methods to reduce or remove the histone contamination without reduction in yield of thylakoids were developed so that true changes in the polypeptide content of thylakoids can be studied during plastid development.
日本植物生理学会の論文