Equilibrium Analysis of Calcium Binding to Cell Walls Isolated from Plant Tissue : STRUCTURE AND FUNCTION OF CELLS

概要

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Primary cell walls were isolated and purified from potato tubers and carrots via a Parr N_2 bomb technique. Calcium binding to purified cell walls was measured with both calcium selective electrode and use of the metallochromic indicator, Arsenazo III. The cell walls used in this study were biologically active and presumably approached the physiological cell wall. Aliquots of the untreated cell walls (control) were then salt-extracted or EDTA-treated and binding properties were compared to the controls. In addition, the binding properties of freshly prepared cell walls were compared to cell walls which were stored for 1 week at 2℃. Both simple Scatchard plot analysis and an electrostatic interaction model were used to evaluate calcium binding parameters. The controls from the two tissue types had inherently different calcium binding properties and these properties were affected by treating the cell walls with salt or EDTA. Cold storage treatment drastically changed the binding properties of carrot cell walls but had negligible effect on potato tuber cell walls.
日本植物生理学会の論文