Diacylglycerol Acyltransferase in Microsomes and Oil Bodies of Oil Palm Mesocarp

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概要

• 論文の詳細を見る

• Specific acyltransferase activities were detected in microsomes and oil bodies from palm mesocarp in the presence of exogenous lysophosphatidic acid, diacylglycerol and glycerol-3-phosphate. These activities were 2-3 times higher per mg protein in oil bodies than in microsomes. Diacylglycerol acyltransferase in both preparations required Mg^<2+>, dithiothreitol, gelatine and fat free bovine serum albumin for maximum activity at pH 8.5. The enzyme had an apparent K_m of 0.18 mM for dipalmitin. In both preparations, the enzyme was active with myristoyl-CoA, palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA. When presented with a mixture of two acyl-CoAs, microsomal enzyme showed no selectivity but oil body enzyme had a preference for oleoyl-CoA over palmitoyl-CoA.

• 日本植物生理学会の論文

著者

• Oo Khaik-cheang

  Department Of Biochemistry University Of Malaya

• Chew Yoke-ha

  Department Of Biochemistry University Of Malaya

関連論文

• Diacylglycerol Acyltransferase in Microsomes and Oil Bodies of Oil Palm Mesocarp

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