Isolation of Genes for Low-Temperature-Induced Proteins in Rice by a Simple Subtractive Method
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概要
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We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5, -9 and - 19. The expression of these genes was slightly stimulated (lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rab16A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lip19 genes were induced 6 h after such a shift.
- 日本植物生理学会の論文
著者
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鈴木 信弘
秋田県立農業短期大学付属生物工学研究所
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SUZUKI Nobuhiro
Akita Prefectural College of Agriculture
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Kusano Tomonobu
Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul
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Aguan Kripamoy
Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agric
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Sugawara Kazuyuki
Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agric
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Suzuki Nobuhiro
Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agric
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Aguan Kripamoy
Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul
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Sugawara Kazuyuki
Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul
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Suzuki Nobuhiro
Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul
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