Variations in Lipid and Fatty Acid Content in Relation to Acetyl CoA Carboxylase in the Marine Prymnesiophyte Isochrysis galbana
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概要
- 論文の詳細を見る
Lipid and fatty acid content was determined in Isochrysis galbana cultures grown under various environmental conditions in steady state continuous cultures. Lipid and・ fatty acid accumulation was observed under severe nitrogen limiting conditions. The rate of in situ lipid synthesis, determined from <14>^C bicarbonate incorporation into lipid fraction, decreased under nitrogen limited growth (μ<0.72 day^<-1>), concomitant with a reduction in the in vitro activity of the enzyme acetyl COA carboxylase. Cellular lipid and fatty acid content remained fairly constant over a wide range of irradiance levels. The rate of lipid synthesis, however, increased as irradiance level was elevated to a maximal value at a light intensity of 150μmol quanta m^<-2>s^<-1> and slightly decreased at a higher photon flux. The in vitro activity of ACCase roughly followed the same pattern of lipid synthesis in response to light intensity. The relative abundance of acetyl COA carboxylase significantly decreased in nitrogen limited cultures grown at low dilution rates (μ<0.72 day^<-1>). A fairly good linear correlation was measured between the cellular content of ACCase and the enzyme activity in cultures grown under nitrogen limiting conditions. Further-more, in nitrogen limited cultures, the cellular fatty acid content was linearly related to the cell capacity to produce malonyl CoA, the end product of ACCase and the building block in fatty acid synthesis.
- 日本植物生理学会の論文
著者
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Livne Alexander
National Institute of Oceanography, Israel Oceanographic and Limnological Research
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Sukenik Assaf
National Institute Of Oceanography Israel Oceanographic And Limnological Research
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Livne Alexander
National Institute Of Oceanography Israel Oceanographic And Limnological Research
関連論文
- Acetyl-Coenzyme A Carboxylase from the Marine Prymnesiophyte Isochrysis galbana
- Variations in Lipid and Fatty Acid Content in Relation to Acetyl CoA Carboxylase in the Marine Prymnesiophyte Isochrysis galbana
- Lipid Synthesis and Abundance of Acetyl CoA Carboxylase in Isochrysis galbana (Prymnesiophyceae) Following Nitrogen Starvation : PROTEINS, ENZYMES AND METABOLISM
- Purification and Characterization of a Light-Harvesting Chlorophyll-Protein Complex from the Marine Eustigmatophyte Nannochloropsis sp. : MEMBRANES AND BIOENERGETICS