Expression of Genes for Photosynthesis and the Relationship to Salt Tolerance of Alfalfa (Medicago sativa) Cells

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概要

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• The basis for the salt tolerant phenotype of a line of Medicago sativa (alfalfa) cells (HG2-N1) derived by selection from a salt sensitive line (HG2) was studied. The salt tolerant HG2-N1 cell line shows eleven fold elevated chlorophyll content over that of the parent salt sensitive HG2 cell line, with an additional two fold increase in chlorophyll levels when the cells are grown in 1% NaCl. In this study, we demonstrate that the chlorophyll accumulation and response to salt was associated with large increases in the two photosynthesis related mRNAs, rbcL (ribulose-1,5-bis-phosphate carboxylase [Rubisco] large subunit) and rbcS (Rubisco small subunit) and a substantial increase in the activity of the holoenzyme. The salinity-induced increase in catalytically competent Rubisco protein in the salt tolerant cell line was highly responsive to light and correlated with the salt tolerant phenotype. In addition, NaCl stimulated rbcL and rbcS mRNA and Rubisco accumulation in dark grown salt tolerant cells, indicating that salt could substitute to some degree for light in stimulating increases in specific mRNA and protein concentrations. In-creased photosynthetic competence associated with these increased protein levels was apparently important in contributing to the salt tolerant phenotype of HG2-N1, since PS II electron trans-port inhibitors (DCMU, cyanazine) were found to significantly reduce the growth of this cell line in the presence of salt, but not in the absence of salt. These results suggest that the salt-induced increase in mRNA and protein accumulation involved in photosynthesis may play a significant role in the salt tolerant capability of HG2-N1 alfalfa cells.

• 日本植物生理学会の論文

著者

• Seemann Jeffrey

  Department Of Biochemistry University Of Nevada

• Seemann Jeffrey

  Department Of Microbiology University Of Nevada

• Winicov Ilga

  Department of Biochemistry, University of Nevada

• Winicov Ilga

  Department Of Biochemistry University Of Nevada

関連論文

• Carboxyarabinitol 1-Phosphate Phosphatase from Leaves of Phaseolus vulgaris and Other Species
• Levels of Carboxyarabinitol 1-Phosphate and Related Photosynthetic Components during Leaf Development in Phaseolus vulgaris : GROWTH AND DEVELOPMENT : PROTEINS, ENZYMES AND METABOLISM
• Expression of Genes for Photosynthesis and the Relationship to Salt Tolerance of Alfalfa (Medicago sativa) Cells
• ^CO_2 Labeling Studies of 2-Carboxyarabinitol 1-Phosphate Synthesis

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