Reinstatement of Microtubule Arrays from Cortical Nucleating Sites in Stomatal Complexes of Lolium rigidum Following Depolymerization of Microtubules by Oryzalin and High Pressure

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Immunofluorescence visualization of microtubule (MT) arrays in stomatal complexes of Lolium rigidum shows that disassembly of the arrays can be successfully achieved using oryzalin or high pressure treatments. Under conditions allowing for MT recovery, MTs reappear within an hour after oryzalin or within 5 min after high pressure treatment. During recovery guard mother cells (GMCs) nucleate MTs at sites distributed randomly in the cell cortex. Even after 22 h of recovery the MTs are not arranged into any configuration found in untreated tissue. This inability to reorganize their MTs after treatment makes GMCs more sensitive to the loss of MTs than are other cells of the leaf. In guard cells (GCs) MTs reappear around the pore at the junction of the periclinal and ventral walls. They subsequently appear throughout most of the cell cortex and the majority of stomatal complexes recover fully organized MT arrays in-distinguishable from those in untreated cells. The results support and extend ultrastructural and immunofluorescence observations that suggest that MTs in GCs of developing stomata are nucleated in the cell cortex.
日本植物生理学会の論文