Plant Regeneration from Isolated Microspore of Indica Rice
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概要
- 論文の詳細を見る
Isolated microspores of rice (Oryza sativa L.) cultivars, IR36 and IR43, belonging to the recalcitrant indica subspecies were cultured. Two types of microspores were observed after isolation from the fresh anthers and from pre-cultured anthers-one type consisted of vacuolated, larger-sized grains, while the other was composed of microspores of smaller sizes with dense cytoplasm. Within few days in culture, all the smaller sized grains were dead, and only the large grains were viable and produced pollen embryos. After 30 days from culture, microcalli were transferred to semisolid modified Murashige and Skoog medium containing 1 mg/liter each of kinetin and naphthaleneacetic acid and kept under continuous light at 25℃. IR36 showed only cell division while IR43 gave 32 green plants from these experiments.
- 日本植物生理学会の論文
著者
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Cho Moon
Horticulture Dept College Of Agriculture Taegu University
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Zapata Francisco
Tissue Culture Laboratory Plant Breeding Department International Rice Research Institute