Potato Gene X^i Confers Inoculum Dependent Resistance to Potato Virus X Replication in Protoplasts

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概要

• 論文の詳細を見る

• Protoplasts were obtained from genetically related Argentine potato (Solanum tuberosum tuberosum) cultivars: Serrana INTA and Huinkul MAG and from an European cultivar: Spunta. Serrana INTA is resistant to potato virus X (PVX) infection because it carries the major reaction gene X^i (believed to be the same as Rx_<acl>) while Huinkul MAG and Spunta are susceptible. Protoplasts were inoculated with a purified preparation of PVXcp (a South American isolate) and then assayed for PVX concentration at different times postinfection by enzyme-linked immunosorbent assay (ELISA), immunofluorescence and nucleic acid hybridization. PVX multiplication rates in Serrana INTA were about fifteen times slower than in susceptible genotypes when cell-bound virus levels just after infection were in the range of 0.01 to 0.1 ng PVX per viable protoplast. However, when inoculum levels were raised to 1 ng PVX per viable protoplast, PVX multiplication was about the same in all three genotypes. To rule out genetic background effects in this behaviour, protoplasts of an Argentine S. acaule clone (PI: 320277) likely carrying the same resistance gene, were infected with PVX in similar conditions, reproducing those results obtained with Serrana INTA. The comparison of PVX replication in protoplasts and whole plants indicate that although X^i gene confers resistance at the cell level it necessitates of tissue structure to fully express immunity.

• 日本植物生理学会の論文
• 1990-09-00

著者

• Hopp H

  Cicv Pcia. De Buenos Aires Arg

• Hopp H.

  Instituto De Biologia Molecular

• Saladrigas M.

  Instituto de Biologia Molecular CIC V-INTA Castelar

• Ceriani M.

  Instituto de Biologia Molecular CIC V-INTA Castelar

• Tozzini Alejandro

  Catedra de Genetica, Facultad de Agronomia, Universidad de Buenos Aires

• Arese Alicia

  Instituto de Biologia Molecular CIC V-INTA Castelar

関連論文

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• Molecular Characterization of a Putative Sucrose : Fructan 6-Fructosyltransferase (6-SFT) of the Cold-Resistant Patagonian Grass Bromus pictus Associated With Fructan Accumulation Under Low Temperatures
• Potato Gene X^i Confers Inoculum Dependent Resistance to Potato Virus X Replication in Protoplasts

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