Effect of Temperature, Light, Nutrients and Dehardening on Abscisic Acid Induced Cold Hardiness in Bromus inermis Leyss Suspension Cultured Cells
スポンサーリンク
概要
- 論文の詳細を見る
The effects of culture conditions on abscisic acid (ABA)-induced freezing tolerance were determined in smooth bromegrass (Bromus inermis Leyss cv. Manchar) cell suspension cultures. Bromegrass cultures initiated with 2 g fr wt of cells achieved maximum freezing tolerances (greater than -32℃) at 25 to 30℃ in the presence of 75 to 100 μM ABA. High levels of freezing tolerance induced by ABA were correlated with high growth rates at 25 and 30℃. In control cells, incubation at 10℃ induced optimum levels of hardiness with minimal growth. Prolonged exposure (6 weeks) of cells to 3℃, with or without ABA, increased freezing tolerance only by several degrees. Exogenous ABA concentrations greater than 100μM were not inhibitory to growth. Repeated exposure to ABA, however, retarded growth and made the cells tolerant to temperatures below -40℃. Removal of ABA from the medium resulted in dehardening of the cells both at 25 and 3℃. Nitrogen had a marginal effect on ABA-induced hardening at 25℃, but inhibited age-dependent hardening of control cell cultures. Light had no effect on the freezing tolerance of cultured cells. Addition of 10% sucrose, 30 min prior to freezing, to bromegrass cells treated with ABA for 4 days increased freezing tolerance more than 15℃. These observations are discussed in relation to the contrasting behaviour of the low temperature and photoperiod dependent cold acclimation of plants.
- 日本植物生理学会の論文
著者
-
ISHIKAWA Masaya
Department of Internal Medicine, Nippon Medical School
-
Robertson A
Univ. Saskatchewan Sk Can
-
Ishikawa Masaya
Department Of Agricultrural Chemistry And Department Of Forestry Faculty Of Agriculture Ehime Univer
-
Ishikawa Masaya
Department Of Cenetic Resources National Institute Of Agrobiological Resources
-
Robertson Albert
Department of Cenetic Resources, National Institute of Agrobiological Resources
-
Gusta Lawrence
Department of Cenetic Resources, National Institute of Agrobiological Resources
-
Gusta Lawrence
Department Of Cenetic Resources National Institute Of Agrobiological Resources
関連論文
- Effects of a Pure α/β-Adrenergic Receptor Blocker on Monocrotaline-Induced Pulmonary Arterial Hypertension With Right Ventricular Hypertrophy in Rats
- PJ-076 Importance of Systolic Blood Pressure in Acute Heart Failure Syndromes : Relationship between Forrester's Classification and Clinical Scenarios(PJ013,Cardiac Function (Clinical) 1 (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of
- Synthesis of hollow silica microparticles from bacterial templates
- Participation of β-Myrcene in the Susceptibility and /or Resistance of Pine Trees to the Pine Wood Nematode, Bursaphelenchus xylophilus(Pesticide Chemistry)
- Intensity Changes in ^1H-NMR Micro-images of Plant Materials Exposed to Subfreezing Temperatures
- β-Myrcene, a Potent Attractant Component of Pine Wood for the Pine Wood Nematode, Bursaphelenchus xylophilus(Pesticide Chemistry)
- Effect of Temperature, Light, Nutrients and Dehardening on Abscisic Acid Induced Cold Hardiness in Bromus inermis Leyss Suspension Cultured Cells
- Ice-Nucleating Substances of Plant Origin