Light Activation of Pyruvate, Orthophosphate Dikinase in Maize Mesophyll Chloroplasts: A Role for Adenylate Energy Charge

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概要

• 論文の詳細を見る

• Pyruvate, orthophosphate dikinase (EC 2.7.9.1) was activated in the light and inactivated following a dark treatment in intact maize mesophyll chloroplasts. Addition of catalase (100-250 units/ml) to the assay medium was necessary to obtain good activation and to keep the enzyme in an active state during illumination. Arsenate and carbonyl cyanide m-chlorophenylhydrazone, uncouplers of photophosphorylation, inhibited the activation. Pyruvate, which has been proposed to have a critical role in supporting the light activation of pyruvate, orthophosphate dikinase, actually inhibited the activation. The pyruvate level in the chloroplast suspension decreased when the enzyme was light-activated. Measurements of adenylates and pyruvate in the chloroplasts indicated that the energy state of the chloroplasts was more important for the light activation than was the level of pyruvate.

• 日本植物生理学会の論文

著者

• Nakamoto H

  Saitama Univ. Urawa Jpn

• Nakamoto Hitoshi

  Radioisotope Center The University Of Tokyo:(present)department Of Biochemistry Faculty Of Science S

• Young Park

  Radioisotope Centre The University Of Tokyo

関連論文

• Changes in the Rate of Photosynthesis during Grain Filling and the Enzymatic Activities Associated with the Photosynthetic Carbon Metabolism in Rice Panicles
• Light Activation of Pyruvate, Orthophosphate Dikinase in Maize Mesophyll Chloroplasts: A Role for Adenylate Energy Charge

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