Decreased Cellulase Activity in Avocado Fruit Subjected to 2.5% O_2 Correlates with Lower Cellulase Protein and Gene Transcript Levels

スポンサーリンク

概要

• 論文の詳細を見る

• Low (2.5%) oxygen atmosphere was previously shown to prevent the normal rise in cellulase activity in avocado fruits primed for ripening by pre-exposure to 10 μl/liter ethylene (Kanellis et al. 1987, Kanellis et al. 1989b). Such primed avocado fruits were used in the present study to determine the effect of 2.5% O_2 atmosphere on the accumulation of cellulase protein and its mRNA using specific antibody and cDNA probes. Decrease in cellulase activity was paralleled by a decrease in immunogenic cellulase protein in fruits held in 2.5% O_2. Under these conditions only 10-20% of total cellulase gene transcripts were expressed. Inclusion of ethylene in the low O_2 atmosphere did not significantly alter the expression of the cellulase gene. Accumulation of cellulase mRNA during ripening was not due to polyadenylation of pre-existing mRNAs. Thus, low O_2 appears to regulate cellulase gene expression at a pre-translational level.

• 日本植物生理学会の論文

著者

• Mehta A

  Ars Usda Md Usa

• Mattoo A

  Ars Usda Md Usa

• Kanellis A.K

  Department of Horticulture, University of Maryland, College Park

• Solomos T

  Department of Horticulture, University of Maryland, College Park

• Mehta A.M

  Plant Hormone Laboratory, USDA-ARS, Beltsville Agricultural Research Center (W)

• Mattoo A.K

  Plant Molecular Biology Laboratory, USDA-ARS, Beltsville Agricultural Research Center (W)

• Solomos T

  Univ. Maryland Md Usa

• Kanellis A.k

  Department Of Horticulture University Of Maryland College Park:(present)institute Of Molecular Biology And Biotechnology

関連論文

• Decreased Cellulase Activity in Avocado Fruit Subjected to 2.5% O_2 Correlates with Lower Cellulase Protein and Gene Transcript Levels

スポンサーリンク