Changes in the Polypeptide Composition during the Ontogenetic Development of the Shoot Apex of Chrysanthemum segetum L. Analyzed by Two-Dimensional Mini-Gel Electrophoresis

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In Chrysanthemum segetum, a quantitative long-day plant grown under long-day conditions, three Stages of ontogenic development were characterized by scanning electron microscopy and two-dimensional mini-gel electrophoresis. Ten μg of protein and silver-staining allowed the detection of 542 different polypeptidic spots, more densely distributed in the acidic region of the gel than in other regions. In the prefloral meristem, in a comparison with the vegetative shoot apex, 10 new polypeptides were identified and 2 polypeptides, unique to the shoot apex, were no longer detectable. In the reproductive meristem, 4 new spots were identified and 2 spots were missing, one of which was present in both the vegetative and prefloral meristems and the other which was specific to the prefloral meristem. The major qualitative changes in the population of polypeptides occurred, in the transition to flowering, during the prefloral stage which has previously been identified as a point of no return in ontogenetic development.