Supramolecular Assembly and Function of Subunits Associated with the Chlorophyll a-b Light-Harvesting Complex II (LHC II) in Soybean Chloroplasts

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The chlorophyll (Chl) a-b light harvesting complex II (LHC II) contains more than 80% of the light-harvesting pigments of photosystem II (PS II) in chloroplasts. The supramolecular assembly and function of this auxiliary antenna system was investigated in Chl b-deficient and Chl b-less mutant chloroplasts from soybean and barley plants, and in their wild-type counterparts. Four distinct LHC II polypeptides were resolved by SDS-PAGE (subunits a, b, c and d), having apparent molecular masses of 29, 28, 27.2 and 26.8 kDa, respectively. The analysis of LHC II subunit composition in different developmental stages of the PS II unit in soybean (3 < Chl a/Chl b<6), indicated the association of specific subunits with the LHC II-inner and LHC II-peripheral in the chloroplast. The amount of subunit a in PS II was constant over a broad range of Chl a/Chl b ratios, suggesting that this subunit is closely associated with the PS II-core complex. Subunit d also appeared to be constant over a wide range of Chl a/Chl b ratios, suggesting close association with the LHC II-inner. The PS II content in subunits b and c in-creased with the PS II antenna development in soybean but the ratio of b/c remained constant in all developmental stages and equal to 2 : 1. Subunit a was present in the Chl b-less chlorina f2 mutant of barley grown under continuous illumination but was absent under intermittent illumination. The results suggest that each subunit binds 13-15 Chl molecules. A working hypothesis is presented on the PS II antenna development and LHC II subunit composition in soy-bean chloroplasts.
日本植物生理学会の論文

Supramolecular Assembly and Function of Subunits Associated with the Chlorophyll a-b Light-Harvesting Complex II (LHC II) in Soybean Chloroplasts

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