INTRACELLULAR LOCALIZATION OF NATIVE AUXIN IN AVENA COLEOPTILE
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概要
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Intracellular localization of the native auxin in the Avena coleoptile tip was investigated by separating cellular fractions by differential centrifugation. Each fraction was extracted with ether and the auxin activity was measured by the sensitized Avena curvature test. After the removal of the native free auxin, each fraction was alkaline-hydrolyzed, and from these hydrolyzates the bound auxin was extracted with ether and its activity was measured. Both the native free auxin and the native bound auxin in these extracts were identified as IAA by paper chromatography. The results show that the native free auxin occurs only in the supernatant soluble cytoplasm, and that the native bound auxin localizes also in the supernatant. The distribution of the externally applied IAA was also investigated.
- 日本植物生理学会の論文
著者
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Ishii Hiroyoshi
Inslitute Of Biology College Of Genelal Education University Of Tokyo
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NAKAMURA TERUKO
Inslitute of Biology, College of Genelal Education, University of Tokyo
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YAMAKI TOSHIO
Inslitute of Biology, College of Genelal Education, University of Tokyo
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Yamaki Toshio
Inslitute Of Biology College Of Genelal Education University Of Tokyo
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Nakamura Teruko
Inslitute Of Biology College Of Genelal Education University Of Tokyo
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Nakamura T.
Inslitute of Biology, College of Genelal Education, University of Tokyo