Ion Content of the Halotolerant Alga Dunaliella salina

概要

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The intracellular concentration of the major ions in Dunaliella salina cells were determined, following the removal of extracellular ions by ion-exchange minicolumns. Log phase cells, grown in media containing 1-4 molar NaCl, contained 30-50 mM Chloride and 200-350 mM magnesium (5 mM in medium). Phosphorus, which is present intracellularly mostly as polyphosphate, was present in amounts of 60-100 fmoles per cell, equivalent to a concentration of 600-1,000 mM (0.2 mM in medium). Previous data indicated that such cells contained 20-40 mM Na^+, 150-300 mM K^+, 20 mM SO^<-2>_4, and very low concentrations of Ca^<2+> and charged nitroge-nous compounds. Mg^<2+> and K^+ seem to serve as the major counter ions for the intracellular negative charge present in the massively accumulated polyphosphates. The former accounts for about 2/3 of the required positive charge. This is supported by the observation that limitation in the phosphate or K^+ supply in the medium lead to a parallel decrease in the accumulation of intracellular phosphorus, Mg^<2+> or K^+.
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