Electrical Characteristics of the Tonoplast of Chara corallina : A Study Using Permeabilised Cells

概要

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Use of permeabilised cells of Chara corallina provides a unique opportunify to study the electrical characteristics of the tonoplast whilst being able to control ionic conditions on the outside of the membrane. Current-voltage (I/V) analysis over wide voltage spans, and admittance measurements at 5 Hz showed that many permeabilised cells had a similar conductance and capacitance to the tonoplast of intact cells. Cells developed two regions of negative-slope conductance upon addition of external Cl^-, which suggests the existence of potential-dependent Cl^- channels in the Chara tonoplast. With Cl^- concentrations similar to those expected in vivo, the resting potential was more sensitive to changes in external K^+ than Cl^-; however, a decrease in external K^+ did not significantly alter the shape of the I/V relation.
日本植物生理学会の論文