Metabolism of Glycerolipids in Plastidial and Extraplastidial Membranes of Etiolated Wheat Leaves : In Vivo and In Vitro Studies

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Etiolated wheat leaves were fed with [1-^<14>C]-acetate and chase experiments were performed in the dark or under light. In both conditions, in extraplastidial membranes, phosphatidylglycerol (PG) and phosphatidylcholine (PC) were fatty acid donors, PG and PC providing palmitate and oleate respectively. The label of linoleate increased only in phosphatidylethanolamine (PE). In etioplasts, PG was also a palmitate donor but PC, rather poor in labelled oleate, was an oleate acceptor, contrary to what was observed in chloroplasts. The galactolipids and sulfoquinovosyldiacyl-glycerol (SL) remained poorly labelled. When isolated etioplasts were labelled in vitro, during the first two hours they incorporated the same amount of [1-^<14>C]-acetate in their phospholipids, whether they were in the presence or in the absence of extraplastidial membranes. Afterwards, the addition of a mitochondrial fraction enhanced the label of PG, mainly in palmitate, then in oleate, and to some extent, and only under light in palmitoleate and linoleate. The mitochondrial fraction might be regarded here as a supplier of labelled precursor to the etioplasts which rapidly accumulated radioactivity in palmitoyl-PG. In PC of isolated etioplasts, only palmitate was fairly labelled. The deficiency in labelled oleoyl-PC in plastids of dark-grown plants and of linoleoyl-PC in extraplastidial membranes might be the reason for the delay in the labelling of unsaturated galactolipids.
日本植物生理学会の論文

Metabolism of Glycerolipids in Plastidial and Extraplastidial Membranes of Etiolated Wheat Leaves : In Vivo and In Vitro Studies

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